Structural analyses of the chromatin remodelling enzymes INO80-C and SWR-C.
نویسندگان
چکیده
INO80-C and SWR-C are conserved members of a subfamily of ATP-dependent chromatin remodelling enzymes that function in transcription and genome-maintenance pathways. A crucial role for these enzymes is to control chromosomal distribution of the H2A.Z histone variant. Here we use electron microscopy (EM) and two-dimensional class averaging to demonstrate that these remodelling enzymes have similar overall architectures. Each enzyme is characterized by a dynamic 'tail' domain and a compact 'head' that contains Rvb1/Rvb2 subunits organized as hexameric rings. EM class averages and mass spectrometry support the existence of single heterohexameric rings in both SWR-C and INO80-C. EM studies define the position of the Arp8/Arp4/Act1 module within INO80-C, and we find that this module enhances nucleosome-binding affinity but is largely dispensable for remodelling activities. In contrast, the Ies6/Arp5 module is essential for INO80-C remodelling, and furthermore this module controls conformational changes that may couple nucleosome binding to remodelling.
منابع مشابه
Structural analyses of the chromatin remodeling enzymes INO80-C and SWR-C
INO80-C and SWR-C are conserved members of a subfamily of ATP-dependent chromatin remodeling enzymes that function in transcription and genome-maintenance pathways. A crucial role for these enzymes is to control chromosomal distribution of the H2A.Z histone variant. Here we use electron microscopy (EM) and two-dimensional (2D) class averaging to demonstrate that these remodeling enzymes have si...
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*Correspondence to: Craig L Peterson; Email: [email protected] Submitted: 06/03/2013; Accepted: 06/03/2013 http://dx.doi.org/10.4161/cc.25704 Comment on: Watanabe S, et al. Science 2013; 340:195-9; PMID:23580526; http://dx.doi.org/10.1126/science.1229758 Organization of eukaryotic genomes into compact, nucleoprotein fibers is key for stuffing a lot of DNA into a tiny nucleus, but thes...
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عنوان ژورنال:
- Nature communications
دوره 6 شماره
صفحات -
تاریخ انتشار 2015